Over
the course of the past decade, there have been remarkable advances in
the study of human pathogenic fungi. These developments have taken place
throughout a wide range of disciplines, and have come as the result of
newly available genome sequences of pathogens such as candida albicans
and other model fungi. In Candida Albicans: Methods and Protocols,
expert researchers explore these exciting new insights, focusing on the
study of medically important fungi and Candida spp in particular.
Chapters examine critical aspects of molecular methods, providing
information on reporter gene assays, transformation, gene expression in
vivo, and methods for large-scale gene disruption. At the same time, the
work includes in-depth descriptions of disease models of candidiasis,
facts about strain identification, and guidelines on the preparation of
samples for proteomic investigations and tandem affinity purification.
Composed in the highly successful "Methods in Molecular BiologyTM"
series format, each chapter contains a brief introduction, step-by-step
methods, a list of necessary materials, and a Notes section which shares
tips on troubleshooting and avoiding known pitfalls. Authoritative and
cutting edge, Candida Albicans: Methods and Protocols is an invaluable
source of methods for investigators in the exhilarating fields of
medical and molecular mycology.
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